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Introducing RESiVATE™ – a new generation of xeno-free cell culture media designed for the rapid expansion of human mesenchymal stem cells (MSCs). Captivate Bio’s RESiVATE™ MSC Expansion Medium is a growth factor rich culture system that enables scientists the ability to customize culture conditions on the fly. Available with or without the supplement, the RESiVATE™ MSC Basal Medium is an optimized baseline formulation that does not contain proteins or unknown supplements, leaving you in full control of your experimental conditions.
Expansion of human bone marrow derived mesenchymal stem cells (BM-MSCs) in RESiVATE MSC Expansion Media is comparable to FBS-containing media in terms of morphology and growth characteristics. Data shown provided by EverCell Bio, a leading stem cell services organization located in Natick, MA, USA.
Results. Expansion of BM-MSCs cultured in RESiVATE MSC Expansion Medium. MSCs were seeded at 8,000 cells/cm2 on day 0 with image captured on day 1 and day 5 during initial passage. Typical “shoalike” morphology of MSCs was observed. Graph: Isolated BM-MSCs were cultured in RESiVATE MSC Expansion Medium or FBS-containing media on fibronectin for 5 passages. Cell counts and viability was obtained using live/dead stain.
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