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Balanced Salt Solutions

Balanced Salt Solutions

Balanced salt solutions including PBS, DPBS, and HBSS are formulated for the irrigation, washing, and the dilution of a variety of cell types which are designed to provide a pH buffering system and maintaining the osmotic balance when using a cell culture medium. Captivate Bio balanced salt solution’s essential function is to maintain pH and osmotic balance as well as provide your cells with water and essential inorganic ions. Captivate Bio salt solutions are available standard formats as well as custom formulations and formats. Please see below for our common liquid products.

captivate bio salt solutions dpbs bottle

Balanced Salt Solutions Overview

We offer ready-to-use salt solutions as well as powdered and liquid formulations to fit your setup and budget.

PBS

PBS is a water-based salt solution with a simple formulation. It is isotonic and non-toxic for most cells. It includes sodium chloride and phosphate buffer and is formulated to prevent osmotic shock while maintaining living cells’ water balance.

DPBS

DPBS includes potassium chloride which is slightly different than PBS. DPBS is available in a larger variety of formulations and is used in biological applications, water-based and includes sodium chloride and phosphate buffer.

HBSS

HBSS is used as buffer systems in cell culture media, HBSS helps maintain optimal physiological pH. Because HBSS is low in reactivity, it is often used atmospheric conditions and may be used to maintain osmolality and pH in biological applications.

Selection Guide

Application
Component(s)
PBS
DPBS
HBSS

Cell adhesion, use when cells remain attached to culture substrate

with Calcium & Magnesium

 

X

X

without Calcium & Magnesium

X

X

Cell survival support added to cultures when medium is absent

without Pyruvate

 

 

X

Measures cell environments, pH inhibitor

with Phenol Red

   

X

without Phenol Red

X

X

X

Stabilizes pH in CO2 environments

with Sodium Bicarbonate

   

X

3 Items

Product List

Set Descending Direction
per page
  1. DPBS, no calcium, no magnesium
    DPBS, no calcium, no magnesium
    DPBS is used for many cell culture applications including washing cells before dissociation, transporting cells and tissue samples, diluting cells for counting, and preparing cell culture reagents. This formulation is without calcium and magnesium.
  2. HBSS, calcium and magnesium
    HBSS, calcium and magnesium
    Captivate Bio's HBSS is a solution used in a variety of cell culture applications including washing, diluting, and transporting cell or tissue samples. This formulation is with calcium and magnesium which is generally used as transport media or for reagent preparation.
  3. PBS 1X, no calcium, no magnesium
    PBS 1X, no calcium, no magnesium
    PBS 1X is a buffered balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Custom Media Services

If you can’t find what you need, our custom services team is here to help. We will work with you to deliver media and reagents that meet your requirements, timeline, and budget. Whether it’s as simple as a change in formulation, bulk small molecules or cytokines, or a completely new product, take advantage of our custom services to move your research forward. Contact our team today to get started.