Research Spotlights
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Human pluripotent stem cells (hPSC), including induced pluripotent stem cells (hiPSC), are a valuable resource for research and cell therapy applications. Successful culture of these cells in vitro requires a cell culture environment that effectively mimics the complexity of the natural extracellular matrix (ECM) while preserving the integrity of the hPSC cultures in terms of pluripotency and functionality.
A common matrix used for hPSC culture is Matrigel®, a basement membrane component derived from mouse EHS tumors. Though widely used since the first publication of mTeSR® media in 2006, Matrigel is far from an ideal substrate, as it is animal-derived, undefined, difficult to handle, and is highly inconsistent in both composition and concentration.
An ideal hPSC culture environment is suitable for both fundamental research and downstream cell therapy applications, with high consistency lot-to-lot and reliable performance over time. myMATRIX™ iPSC pre-coated plates provide a chemically defined ECM substrate that produces high-quality hPSC cultures and reproducible experimental results. myMATRIX iPSC pre-coated plates are compatible with common hPSC culture media and protocols, including mTeSR media, and both clump and single-cell passaging techniques, offering an easy transition from Matrigel to a hPSC culture system that meets today’s basic scientific standards.
Research area: Maintenance culture, expansion scale-up, and cloning of human pluripotent stem cells
Cell type(s): hPSCs, hiPSCs, and hESCs
Experiment purpose: A demonstration of performance of myMATRIX iPSC pre-coated plates as a suitable or superior alternative to Matrigel. Standard hiPSC culture conditions and techniques were compared using Matrigel and myMATRIX iPSC to show that myMATRIX iPSC plates are interchangeable with common media (mTeSR1) and workflows (clump and single-cell passaging, cell expansion, freeze/thawing, and cloning).
Product highlights: myMATRIX iPSC pre-coated cultureware is a novel chemically defined, and xeno-free biomatrix. Proven ability to maintain healthy, pluripotent stem cells in long-term culture and customizable options to match scale-up workflows.
References: Segeletz, S. et al. 2021. White Paper No. 472. denovoMATRIX.
myMATRIX iPSC pre-coated plates are a reliable, xeno-free, and chemically defined alternative to Matrigel, with equal or superior attachment, proliferation, growth speed, and maintenance of iPSC stem-ness and differentiation potential, while maintaining characteristic morphology and genetic integrity over time.
Additional to these demonstrated features, myMATRIX iPSC pre-coated plates are an easy-to-use, highly consistent biomatrix that contributes to reliable and reproducible results over time, making this an excellent platform for both fundamental cell research and iPSC scale-up for cell therapy and regenerative medicine applications.
Want to know more about myMATRIX iPSC surfaces?Download our Research Spotlight to learn more about this white paper.
Image source: Segeletz, S. et al. 2021. White Paper No. 472. denovoMATRIX.
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