Accutase

Accutase® Cell Detachment Solution contains proteolytic and collagenolytic enzymes for cell detachment. A sterile filtered ready-to-use solution specifically developed for the gentle and routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.

Accutase performs exceptionally well in detaching primary and stem cells and maintaining high cell viability compared to animal origin enzymes such as trypsin. Some downstream applications following Accutase treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Cell lines tested for Accutase application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells and primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, and established cell lines such as adherent CHO and BHK cells and 293 cells. Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Features

• Efficient, with gentle detachment of adherent cells within minutes
• An alternative to Trypsin
• Able to eliminate neutralization or wash steps
• Accutase does not contain mammalian or bacterial derived products