Compound E

Small Molecules for Stem Cell Research

Compound E is a potent γ-secretase inhibitor that specifically blocks β-amyloid-40 (IC50 of 0.24), β-amyloid-42 (IC50 of 0.37), and cleavage of the Notch domain (IC50 of 0.32). Compound E has been shown to promote the differentiation of embryonic stem cells (ESCs) to primitive neural precursors in addition to generating a self-renewing progenitor neuroepithelia stem cell population (Li, et al.) when combined with CHIR99021, SB431542, and hLIF. Forebrain GABA interneurons have also been derived from human pluripotent stem cells (PSCs) through the utilization of Compound E (Liu, et al.); additionally, it has shown promising effects in Alzheimer’s disease models using iPSC-derived neurons (Yagi, et al.).

Alternative names: XXI, Compound E (secretase inhibitor), DuPont E, C-E, γ-secretase inhibitor XXI, Gammasecretase inhibitor XXI, gamma inhibitor
Applications:enhances growth inhibition, differentiation of hPSCs
Cell types: neural, hPSCs, hESCs

Keywords: Compound E, gamma-secretase, g-secretase, neuronal differentiation, neurons, self renewal, notch pathways, neural stem cells, hPSCs

Documents

• Technical Data Sheet
• SDS
• Brochure: Small Molecules for Stem Cell Research
• FAQs: General Guide for Small Molecules

References and Publications

1. Li, et al. 2011. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. PNAS. 108(20): 8299-304.
2. Liu, et al. 2013. Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells. Nature Protocols. 8: 1670-1679.
3. Yagi, et al. 2011. Modeling familial Alzheimer’s disease with induced pluripotent stem cells. Human Molecular Genetics. 20(23): 4530-4539.