DMEM/F12 Medium, HEPES

DMEM/F12 Medium is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham's F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. DMEM/F12 is a basal cell culture media that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

Cells cultured in DMEM/F12 with HEPES include mouse embryonic stem cell, epithelial cells, neural stem cells, human embryonic stem cells, mesenchymal stem cells, induced pluripotent stem cells, and hematopoietic stem cells.

This DMEM/F12 is modified as follows:

• L-Glutamine
• HEPES

• Phenol Red

Standard packaging includes 500 mL and 1000 mL bottles. Other volumes and bioprocessing bags are available upon request. To inquire about bulk or custom options, please inquire here

Documents

• Product Information Sheet

References and Publications

1. Morton, H.J. 1970. A survey of commercially available tissue culture media. In Vitro 6(2):89.
2. Ham, R.G. 1965. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
3. Smith, J.D., et al., 1960. The nucleic acid of polyoma virus. Virology 12:185.
4. Dulbecco, R., et al. 1959. Plaque formation by the polyoma virus. Virology 8:396.