DMEM/F12 Medium

DMEM/F12 Medium is a 1:1 mixture of DMEM and Hams F-12. The combination of DMEM’s high concentration of amino acids, vitamins, and glucose, enriched with Ham's F-12 Nutrient Mixture, was found to support the growth of a wide range of mammalian cells. DMEM/F12 is a basal cell culture media that requires supplementation with serum like FBS, growth factors, or human platelet lysate.

Cells cultured in DMEM/F12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

This DMEM/F12 is modified as follows:

• L-glutamine
• Phenol Red

• HEPES

Documents

• Product Information Sheet

References and Publications

1. Morton, H.J. 1970. A survey of commercially available tissue culture media. In Vitro 6(2):89.
2. Ham, R.G. 1965. Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.
3. Smith, J.D., et al., 1960. The nucleic acid of polyoma virus. Virology 12:185.
4. Dulbecco, R., et al. 1959. Plaque formation by the polyoma virus. Virology 8:396.