MCDB 131, no L-glutamine

MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.

Recent studies have shown MCDB 131 to be effective in definitive endoderm differentiation of human pluripotent stem cells (hPSCs), allowing for the derivation of cell lines that produce epithelial lining of systems such as the intestines, lungs, liver, and pancreas. In addition, MCDB 131 has proven to be a critical component in the differentiation of hPSCs into functional pancreatic beta cells through several culture stages producing definitive endoderm, primitive gut tube, pancreatic progenitors, endocrine progenitors, and finally, insulin-producing cells.

Documents

• Product Information Sheet
• Media Formulation