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Human Platelet Lysate (HPL) provides superior cell performance and cell expansion when compared to other supplements such as FBS or chemically defined media. PLTGold® HPL is a revolutionary xeno-free, heparin-free supplement which provides rapid cell expansion with proven success across various cell types including MSCs and immune cells. Available in research and clinical grades, PLTGold® is an excellent supplement to cell culture media and for use in both 2D and 3D culture systems with a focus on research and cell therapy applications.
Figure 2. Real time imaging of adipose-derived MSC growth using PLTMax® or PLTGold® Human Platelet Lysate as a media supplement showed increased cell growth kinetics compared to cells grown in medium supplemented with FBS or Human AB Serum.
Large-scale expansion of cells including MSCs, T-cells, hematopoietic stem cells, iPSCs, tumor cells, and fibroblasts.
Yes, PLTGold is a completely xeno-free supplement that does not require heparin (or used in the manufacturing process). PLTGold has an FDA registered Master File available for reference.
Both grades are manufactured under the same conditions and follow the same protocols. The clinical-grade product includes additional endotoxin testing and batch record documentation. All products have a CofA available.
Yes, with HPL it is possible to add too much to your cultures. We’ve seen that HPL can slow the growth of MSCs at high concentrations. Typically, we recommend using human platelet lysate at 2%-10% concentrations depending on your cell type.
Cells grown in human platelet lysate typically use half the concentration compared to cells cultured in fetal bovine serum. Standard protocols suggest adding 5% concentration. However, we recommend titrating anywhere between 2-10% in order to maximize cell growth and narrow in on the best concentration for your cultures.
Starting at $25.00
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