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Human Platelet Lysate (HPL) provides superior cell performance and cell expansion when compared to other supplements such as FBS or chemically defined media. PLTGold® HPL is a revolutionary xeno-free, heparin-free supplement which provides rapid cell expansion with proven success across various cell types including MSCs and immune cells. Available in research and clinical grades, PLTGold® is an excellent supplement to cell culture media and for use in both 2D and 3D culture systems with a focus on research and cell therapy applications.
Figure 2. Real time imaging of adipose-derived MSC growth using PLTMax® or PLTGold® Human Platelet Lysate as a media supplement showed increased cell growth kinetics compared to cells grown in medium supplemented with FBS or Human AB Serum.
Bhat, S., et al. 2021. Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions. https://doi.org/10.1038/s41598-021-83088-1
Ankrum, JA., et al. 2019. Nature vs. Nurture: Defining the Effects of Mesenchymal Stromal Cell Isolation and Culture Conditions on Resiliency to Palmitate Challenge. Front Immunol. PMID: 31134100
Alonso-Camino, V., et al. 2017. In vitro expansion of mesenchymal stem cells using media supplemented with unfractionated, heparin-free platelet lysate. Volume 19, Issue 5, Supplement, Pages e16-e17.
Burnouf, T., et al. 2016. Human platelet lysate: Replacing fetal bovine serum as a gold standard for human cell propagation. Biomaterials. 2016 Jan;76:371-87. doi: 10.1016/j.biomaterials.2015.10.065. Epub 2015 Oct 28. PMID: 26561934.
Crespo-Diaz, R.et al. 2010. Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. Cell Transplant. 2011;20(6):797-811. doi: 10.3727/096368910X543376. PMID: 21092406
Large-scale expansion of cells including MSCs, T-cells, hematopoietic stem cells, iPSCs, tumor cells, and fibroblasts.
Yes, PLTGold is a completely xeno-free supplement that does not require heparin (or used in the manufacturing process). PLTGold has an FDA registered Master File available for reference.
PLTGold is a completely xeno-free human platelet lysate which contains all essential growth factors for healthy cell growth. Donor units are pooled in large batches and manufactured by Mill Creek Life Sciences under a proprietary method to produce highly consistent and reliable lots.
Both grades are manufactured under the same conditions and follow the same protocols. The clinical-grade product includes additional endotoxin testing and batch record documentation. All products have a CofA available.
Yes, with HPL it is possible to add too much to your cultures. We’ve seen that HPL can slow the growth of MSCs at high concentrations. Typically, we recommend using human platelet lysate at 2%-10% concentrations depending on your cell type.
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