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Y-27632 is a common research tool used in the field of stem cell research for maintenance, differentiation, and the survival of cells following cryopreservation. Y-27632 is used as 3D growth matrix component and included in organoid differentiation media.
Y-27632 dihydrochloride is a highly specific inhibitor of Rho-associated coiled-coil protein kinase (ROCK) activity as a cell-permeable, ATP-competitive inhibitor of both ROCK1 (Ki of 220 nM) and ROCK2 (Ki 300 nM) (Ishizaki, et al.). Human embryonic stem cell (ESC) survival is improved through enhanced attachment following single cell dissociation, cryopreservation, and FACS sorting in both feeder-dependent and -independent cultures (Emre, et al.). This enhanced cell attachment, and thus recovery, is boosted through decreased dissociation-induced apoptosis (Watanabe, et al.). Y-27632 dihydrochloride primes human induced pluripotent stem cells (iPSCs) to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.
Alternative names: Y-27632 dihydrochloride, Y-27632, ROCK inhibitor, Y27632, Rho kinase inhibitor Applications: Differentiation, cryopreservation, expansion, maintenance, reprogramming, spheroid Culture Cell types: Neurons, Pluripotent Stem Cells, iPSCs, ESCs
Keywords: Y-27632 dihydrochloride, Y27632 ROCK inhibitors, rho-kinase, stem cells, organoids, induced pluripotent stem cells, iPSCs, ROCKI, ROCK1, ROCKII, ROCK2, reprogramming, cryopreservation, differentiation, cell freezing, cell culture media, reprogramming, stem cell survival
Y27632 is commonly used to improve cell survival during the routine culture of human pluripotent stem cells (hPSCs), as well as many other sensitive cell types. In addition to routine passaging of hPSCs (both embryonic and induced), common applications include cellular reprogramming/iPSC generation, single cell cloning and gene editing, cryopreservation and recovery, isolation and culture of cancer stem cells, reproductive biology, and transplant medicine.
Y27632 is a small molecule inhibitor of the Rho-associated coiled-coil containing kinase (ROCK) that improves survival of human pluripotent stem cells. Y27632 improves cell survival by blocking the cell contraction that leads to cell death by apoptosis and anoikis by inhibiting the ROCK pathway.
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