JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
The CET Cocktail is comprised of the small molecule components in the CEPT cocktail: Chroman 1, Emricasan, and Trans-IRIB (CET). The CEPT cocktail is a published, optimized combination of targeted small molecules and culture additives proven to enhance stem cell survival and address the key challenges in translational stem cell research. The small molecules in the CET Cocktail provide comprehensive cytoprotection to overcome cellular stressors inherent in stem cell workflows.
This combination of small molecules has been shown to directly and efficiently enhance pluripotent stem cell survival and promote clonal growth and expansion of genetically stable hPSCs.1 The CEPT cocktail allows for safe, controlled, and efficient strategies for the generation, expansion, differentiation, and banking of stem cell-based therapeutic products. This small molecule combination is also key for optimized embryoid body (EB) and organoid formation, as well as superior cryopreservation of pluripotent and differentiated cells by protecting cells from multiple cellular stress mechanisms in a synergistic fashion.1
Alternative names: CE(P)T cocktail, CEPT, cet cocktail molecules Applications: Stem cell survival, CEPT Cocktail, cell translation Cell types: Pluripotent Stem Cells, iPSCs, ESCs, neurons
The CET Cocktail includes the following components:
For best results, prepare the complete CEPT cocktail according to the published recipe, include Polyamine Solution (Sigma P8483) in addition to the CET Cocktail of small molecules in the cell culture media just before use.
References and sample data are from publications that support the biological activity of the product. This data reference is from Chen et al. 2021 Nat Methods.
Figure 1. Cell survival. hPSCs were seeded as single cells at an ultra-low density of just 25 cells/cm2 and incubated with Y27632 and various combinations of the CEPT small molecule and polyamine components, demonstrating combinatorial improvements to cell survival.
Figure 2. Improved clonal growth and expansion of hPSCs. An alkaline phosphatase staining assay of hPSCs cultured with Y27632, the complete CEPT combination, and commercially available CloneR™ supplement, clearly showed more alkaline phosphatase-positive clones in cultures exposed to CEPT.
The data provided herein may relate to products or workflows that have not been fully validated by Captivate Bio and is subject to change without notice.
Keywords: CEPT Cocktail, Chroman 1, Emricasan, Trans-ISRIB, ISRIB, small molecule, stem cell, embryonic, pluripotent, hESC, hiPSC, hPSC, single-cell passaging, cell dissociation, cell stress, ROCK, ROCK inhibitor, reprogramming, cloning, gene editing, cell survival, cell recovery, cryopreservation, freezing, recovery, attachment, NIH, polyamines, neurons, cardiomyocytes, cell function, EDTA, Accutase, cell banking, cell bank, DNA break, cell attachment, cytoprotection, embryoid body, EB, organoid
The P in the published CEPT supplementation method stands for Polyamine Solution, a standard cell culture additive that supports cell growth. The Captivate Bio Polyamine Solution (Cat. No. CCPM08) is available for purchase separately.
The CET Cocktail is composed of the small molecule components of the published CEPT cocktail (Chen et al. Nat Methods 2021). These 3 small molecules, in combination with polyamine supplement, were shown in the study to improve cytoprotection of a variety of hPSC lines. The following cell lines were used in the published study:
While the treatment duration may vary across applications, the small molecules in the CET Cocktail are intended to be used according to the CEPT supplementation strategy for improved human pluripotent stem cell survival and viability in culture. (See Chen et al. Nat Methods 2021 for more information on CEPT.)Generally, the duration of each CET (or CEPT) treatment is approximately 24 hours. Dissociated cells are plated in CET-supplemented media, which is replaced with fresh culture media the following day during the first feeding. Some clonal assays may require up to 3 days of treatment in order not to disturb the clonal colonies with an excessive media change during the initial attachment period.
Yes. Each of the small molecule compounds in the CET Cocktail can be purchased separately: Chroman 1 (SML20A); Emricasan (SML21B); Trans-ISRIB (SML22B). The entire CEPT strategy can be sourced individually as well with the addition of Polyamine Solution (CCPM08).
The CET Cocktail kit contains one vial each of the following small molecules: Chroman 1 (5 mg); Emricasan (10 mg); Trans-ISRIB (10 mg).
Download this free eBook from Captivate Bio. We’ve compiled information over the years to help you build on a lab program designed to fit your own needs.
PDF | Download our Small Molecules for Cell and Gene Therapy Research flyer. Now offering RUO and GMP-grade small molecules for a variety of cell types.
PDF | Download The Captivate Bio Access Program flyer and learn more about how we can support small and mid-size life science companies who are looking for immediate access to consulting, marketing, and sales programs.
