Accutase® Cell Detachment Solution is a ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes for use in dissociation of cells from standard and adhesion-coated plasticware. Ideal replacement for trypsin and for use with stem cells including MSCs and hPSCs.

The CET Cocktail is comprised of the small molecule components in the CEPT cocktail: Chroman 1, Emricasan, and Trans-IRIB (CET). Enhances cell survival and promotes clonal growth of genetically stable hPSCs.
Size: 1 Kit

Highly potent and selective ROCK inhibitor with greater effectiveness at lower doses than Y27632. Enhances cell survival of dissociated hPSCs.

DMEM/F12 Media, with HEPES and L-Glutamine is a basal formulation used for supporting the growth of various stem cells including human ESCs and hiPSCs.

DPBS is used for many cell culture applications including washing cells before dissociation, transporting cells and tissue samples, diluting cells for counting, and preparing cell culture reagents. This formulation is without calcium and magnesium.

IWP 2 is a potent inhibitor of Wnt processing and secretion (IC50 = 27nM). IWP 2 inactivates PORCN, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. IWP-2 has also been used in protocols to reprogram somatic cells to chemically-induced PSCs.

IWP 4 is a potent inhibitor of Wnt/β-catenin signaling (IC50 = 25 nM). Has minimal effect on Notch and Hedgehog signaling pathways. Induces differentiation of cardiomyocytes from human ESCs and iPSCs.

PBS 1X is a buffered balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.

Retinoic Acid is a metabolite of vitamin A that acts as a ligand for nuclear retinoic acid receptors. Retinoic acid has been used for the differentiation of embryonic stem cells into motor neurons and has also played an important role in protocols involving the differentiation of human pluripotent stem cells (PSCs) into functional pancreatic β cells.

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. This formulation is modified with L-glutamine and Phenol Red.